NSJ Bioreagents

CDT1 Antibody / DNA replication factor Cdt1

Product Code:
 
NSJ-RQ7910
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CDT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with CDT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDT1 antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Raji, 4) human K562, 5) rat liver and 6) mouse liver tissue lysate with CDT1 antibody. Predicted molecular weight ~60 kDa, commonly observed at 60-70 kDa.
❮❯

Flow cytometry testing of human HepG2 cells with CDT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDT1 antibody.
Western blot testing of 1) human HeLa, 2) human Jurkat, 3) human Raji, 4) human K562, 5) rat liver and 6) mouse liver tissue lysate with CDT1 antibody. Predicted molecular weight ~60 kDa, commonly observed at 60-70 kDa.

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CodeSizePrice
NSJ-RQ7910-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
CDT1 (Chromatin licensing and DNA replication factor 1) is a protein that in humans is encoded by the CDT1 gene. The protein encoded by this gene is involved in the formation of the pre-replication complex that is necessary for DNA replication. The encoded protein can bind geminin, which prevents replication and may function to prevent this protein from initiating replication at inappropriate origins. Phosphorylation of this protein by cyclin A-dependent kinases results in degradation of the protein.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids P18-L546) was used as the immunogen for the CDT1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9H211