NSJ Bioreagents

CRYAA Antibody / Alpha A Crystallin

Product Code:
 
NSJ-RQ7930
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8H6
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CRYAA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HepG2 cells with CRYAA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CRYAA antibody.
2 / 2
Western blot testing of 1) rat eye, 2) rat C6 and 3) mouse eye tissue lysate with CRYAA antibody. Expected molecular weight: 20-23 kDa.
❮❯

Flow cytometry testing of human HepG2 cells with CRYAA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CRYAA antibody.
Western blot testing of 1) rat eye, 2) rat C6 and 3) mouse eye tissue lysate with CRYAA antibody. Expected molecular weight: 20-23 kDa.

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CodeSizePrice
NSJ-RQ7930-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Alpha-crystallin A chain is a protein that in humans is encoded by the CRYAA gene. Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-S173) was used as the immunogen for the CRYAA antibody.
Purity:
Antigen affinity purified
Uniprot #:
P02489