NSJ Bioreagents

PITPNB Antibody / PI-TP-beta

Product Code:
 
NSJ-RQ7934
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PITPNB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with PITPNB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
IHC staining of FFPE human colon adenocarcinoma tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human testicular seminoma tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE mouse brain tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE rat brain tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human K562, 4) human Raji, 5) rat brain, 6) rat lung, 7) mouse brain and 8) mouse lung tissue lysate with PITPNB antibody. Predicted molecular weight ~32 kDa.
7 / 8
Flow cytometry testing of human HeLa cells with PITPNB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PITPNB antibody.
8 / 8
Flow cytometry testing of human HeLa cells with PITPNB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PITPNB antibody.
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Immunofluorescent staining of FFPE human esophageal squamous cell carcinoma tissue with PITPNB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human colon adenocarcinoma tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human testicular seminoma tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with PITPNB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human K562, 4) human Raji, 5) rat brain, 6) rat lung, 7) mouse brain and 8) mouse lung tissue lysate with PITPNB antibody. Predicted molecular weight ~32 kDa.
Flow cytometry testing of human HeLa cells with PITPNB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PITPNB antibody.
Flow cytometry testing of human HeLa cells with PITPNB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PITPNB antibody.

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CodeSizePrice
NSJ-RQ7934-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Phosphatidylinositol transfer protein beta isoform is a protein that in humans is encoded by the PITPNB gene. This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q148-R170) was used as the immunogen for the PITPNB antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P48739