NSJ Bioreagents

ORP8 Antibody / Oxysterol-binding protein-related protein 8 / OSBPL8

Product Code:
 
NSJ-RQ7942
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ORP8 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with ORP8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) 293T and 2) HEL cell lysate with ORP8 antibody. Predicted molecular weight ~101 kDa.
3 / 3
Flow cytometry testing of human HepG2 cells with ORP8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ORP8 antibody.
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Immunofluorescent staining of FFPE human HeLa cells with ORP8 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) 293T and 2) HEL cell lysate with ORP8 antibody. Predicted molecular weight ~101 kDa.
Flow cytometry testing of human HepG2 cells with ORP8 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ORP8 antibody.

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CodeSizePrice
NSJ-RQ7942-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Oxysterol-binding protein-related protein 8 is a protein that in humans is encoded by the OSBPL8 gene. This gene encodes a member of a family of proteins containing an N-terminal pleckstrin homology domain and a highly conserved C-terminal oxysterol-binding protein-like sterol-binding domain. It binds mutliple lipid-containing molecules, including phosphatidylserine, phosphatidylinositol 4-phosphate (PI4P) and oxysterol, and promotes their exchange between the endoplasmic reticulum and the plasma membrane. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D33-K889) was used as the immunogen for the ORP8 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9BZF1