NSJ Bioreagents

OPLAH Antibody / 5-oxo-L-prolinase / 5-OPase

Product Code:
 
NSJ-RQ7944
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the OPLAH antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human PC-3 cells with OPLAH antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HepG2, 2) human T-47D, 3) monkey COS-7, 4) rat liver, 5) rat brain, 6) mouse liver and 7) mouse brain tissue lysate with OPLAH antibody. Predicted molecular weight ~137 kDa.
3 / 3
Flow cytometry testing of human HEL cells with OPLAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPLAH antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with OPLAH antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human T-47D, 3) monkey COS-7, 4) rat liver, 5) rat brain, 6) mouse liver and 7) mouse brain tissue lysate with OPLAH antibody. Predicted molecular weight ~137 kDa.
Flow cytometry testing of human HEL cells with OPLAH antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OPLAH antibody.

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CodeSizePrice
NSJ-RQ7944-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H788-E1072) was used as the immunogen for the OPLAH antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O14841