NSJ Bioreagents

4E-T Antibody / EIF4ENIF1

Product Code:
 
NSJ-RQ7948
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the 4E-T antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human 293T, 3) human MCF7, 4) human HepG2, 5) rat brain, 6) rat NRK, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with 4E-T antibody. Expected molecular weight ~88/108 kDa (multiple isoforms but can be observed at ~140 kDa (possibly due to phosphorylation or high proline content-Ref 1).
❮❯

Flow cytometry testing of human HL60 cells with 4E-T antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= 4E-T antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human MCF7, 4) human HepG2, 5) rat brain, 6) rat NRK, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with 4E-T antibody. Expected molecular weight ~88/108 kDa (multiple isoforms but can be observed at ~140 kDa (possibly due to phosphorylation or high proline content-Ref 1).

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CodeSizePrice
NSJ-RQ7948-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein that in humans is encoded by the EIF4ENIF1 gene. The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D13-Q985) was used as the immunogen for the 4E-T antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9NRA8