NSJ Bioreagents

PCCB Antibody / Propionyl-CoA carboxylase beta chain

Product Code:
 
NSJ-RQ7949
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PCCB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human colon adenocarcinoma tissue with PCCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human liver cancer tissue with PCCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) human HCCT, 2) rat liver and 3) mouse liver tissue lysate with PCCB antibody. Predicted molecular weight: 58-61 kDa (two isoforms).
4 / 4
Flow cytometry testing of human HepG2 cells with PCCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCCB antibody.
❮❯

IHC staining of FFPE human colon adenocarcinoma tissue with PCCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with PCCB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HCCT, 2) rat liver and 3) mouse liver tissue lysate with PCCB antibody. Predicted molecular weight: 58-61 kDa (two isoforms).
Flow cytometry testing of human HepG2 cells with PCCB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PCCB antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ7949-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D83-L539) was used as the immunogen for the PCCB antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P05166