NSJ Bioreagents

OGDHL Antibody / 2-Oxoglutarate dehydrogenase-like

Product Code:
 
NSJ-RQ7954
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the OGDHL antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with OGDHL antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HepG2, 2) human SH-SY5Y, 3) human U-2 OS, 4) rat brain, 5) rat kidney, 6) mouse brain and 7) mouse kidney tissue lysate with OGDHL antibody. Predicted molecular weight: 91-114 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of human U937 cells with OGDHL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OGDHL antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with OGDHL antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human SH-SY5Y, 3) human U-2 OS, 4) rat brain, 5) rat kidney, 6) mouse brain and 7) mouse kidney tissue lysate with OGDHL antibody. Predicted molecular weight: 91-114 kDa (multiple isoforms).
Flow cytometry testing of human U937 cells with OGDHL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OGDHL antibody.

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CodeSizePrice
NSJ-RQ7954-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorscence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
The protein encoded by this gene is similar to oxoglutarate dehydrogenase (OGDH) of the OGDH complex, which degrades glucose and glutamate. This gene encodes several isoforms, including some that appear to localize to mitochondria. The encoded protein down-regulates the AKT signaling cascade and can suppress the growth of cervical cancer cells.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-F1010) was used as the immunogen for the OGDHL antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q9ULD0