NSJ Bioreagents

GSDMD Antibody / Gasdermin D

Product Code:
 
NSJ-RQ7955
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
6D11
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GSDMD antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE human PC-3 cells with GSDMD antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 6
IHC staining of FFPE human esophageal squamous carcinoma tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human cervical intraepithelial neoplasia tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human cervical cancer tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) ThP-1, 2) SW620, 3) Jurkat, 4) U-87 MG and 5) PC-3 cell lysate with GSDMD antibody. Predicted molecular weight ~53 kDa.
6 / 6
Flow cytometry testing of human Jurkat cells with GSDMD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GSDMD antibody.
❮❯

Immunofluorescent staining of FFPE human PC-3 cells with GSDMD antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human esophageal squamous carcinoma tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical intraepithelial neoplasia tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) ThP-1, 2) SW620, 3) Jurkat, 4) U-87 MG and 5) PC-3 cell lysate with GSDMD antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human Jurkat cells with GSDMD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GSDMD antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ7955-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Gasdermin D is a precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. [UniProt]
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-H484) was used as the immunogen for the GSDMD antibody.
Localisation:
Nucleus, cytoplasm, cell membrane, secreted
Purity:
Antigen affinity purified
Uniprot #:
P57764