NSJ Bioreagents

ORP9 Antibody / OSBPL9

Product Code:
 
NSJ-RQ7958
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ORP9 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with ORP9 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human Jurkat and 2) mouse testis tissue lysate with ORP9 antibody. Predicted molecular weight: 63-84 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of human Raji cells with ORP9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ORP9 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with ORP9 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human Jurkat and 2) mouse testis tissue lysate with ORP9 antibody. Predicted molecular weight: 63-84 kDa (multiple isoforms).
Flow cytometry testing of human Raji cells with ORP9 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ORP9 antibody.

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CodeSizePrice
NSJ-RQ7958-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Oxysterol binding protein-like 9, also called OSBP-related protein 9 (ORP9) is a protein that in humans is encoded by the OSBPL9 gene. This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D27-K652) was used as the immunogen for the ORP9 antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q96SU4