NSJ Bioreagents

OSTF1 Antibody / Oste°Clast-stimulating factor 1

Product Code:
 
NSJ-RQ7959
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the OSTF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with OSTF1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human MCF7, 4) human placenta, 5) rat liver, 6) rat PC-12, 7) mouse liver and 8) mouse C2C12 cell lysate with OSTF1 antibody. Predicted molecular weight ~24 kDa, commonly observed at 24-28 kDa.
3 / 3
Flow cytometry testing of human U-2 OS cells with OSTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OSTF1 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with OSTF1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human MCF7, 4) human placenta, 5) rat liver, 6) rat PC-12, 7) mouse liver and 8) mouse C2C12 cell lysate with OSTF1 antibody. Predicted molecular weight ~24 kDa, commonly observed at 24-28 kDa.
Flow cytometry testing of human U-2 OS cells with OSTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= OSTF1 antibody.

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CodeSizePrice
NSJ-RQ7959-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Osteoclast-stimulating factor 1 is a protein that in humans is encoded by the OSTF1 gene. Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R57-K191) was used as the immunogen for the OSTF1 antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q92882