NSJ Bioreagents

AARS1 Antibody / AlaRS

Product Code:
 
NSJ-RQ7963
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the AlaRS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with AlaRS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) K562, 2) HepG2 and 3) HeLa cell lysate with AlaRS antibody. Predicted molecular weight ~107 kDa.
3 / 3
Flow cytometry testing of human K562 cells with AlaRS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AlaRS antibody.
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Immunofluorescent staining of FFPE human MCF7 cells with AlaRS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) K562, 2) HepG2 and 3) HeLa cell lysate with AlaRS antibody. Predicted molecular weight ~107 kDa.
Flow cytometry testing of human K562 cells with AlaRS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= AlaRS antibody.

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CodeSizePrice
NSJ-RQ7963-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
An aminoacyl-tRNA synthetase (AlaRS, aaRS or ARS), also called tRNA-ligase, is an enzyme that attaches the appropriate amino acid onto its corresponding tRNA. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R729-N968) was used as the immunogen for the AlaRS antibody.
Localisation:
Cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
P49588