NSJ Bioreagents

KIF2A Antibody / Kinesin-like protein KIF2A

Product Code:
 
NSJ-RQ7980
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the KIF2A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
IHC staining of FFPE human placental tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 6
IHC staining of FFPE human lung cancer tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE human pancreatic cancer tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
Immunofluorescent staining of FFPE human SiHa cells with KIF2A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 6
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human K562, 5) rat NRK, 6) mouse lung and 7) mouse NIH 3T3 cell lysate with KIF2A antibody. Predicted molecular weight ~80 kDa, commonly observed at 80-110 kDa.
6 / 6
Flow cytometry testing of human K562 cells with KIF2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIF2A antibody.
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IHC staining of FFPE human placental tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human pancreatic cancer tissue with KIF2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human SiHa cells with KIF2A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human K562, 5) rat NRK, 6) mouse lung and 7) mouse NIH 3T3 cell lysate with KIF2A antibody. Predicted molecular weight ~80 kDa, commonly observed at 80-110 kDa.
Flow cytometry testing of human K562 cells with KIF2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KIF2A antibody.

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CodeSizePrice
NSJ-RQ7980-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Kinesin-like protein KIF2A is a protein that in humans is encoded by the KIF2A gene. The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A2-L706) was used as the immunogen for the KIF2A antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
O00139