NSJ Bioreagents

NXN Antibody / Nucleoredoxin / NXR

Product Code:
 
NSJ-RQ7985
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Nucleoredoxin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human Caco-2 cells with Nucleoredoxin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human placental tissue with Nucleoredoxin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human renal clear cell carcinoma tissue with Nucleoredoxin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) Caco-2, 2) HepG2, 3) HEK293, 4) A549, 5) U-2 OS, 6) A431 and 7) HeLa cell lysate with Nucleoredoxin antibody. Predicted molecular weight ~48 kDa.
5 / 5
Flow cytometry testing of human SiHa cells with Nucleoredoxin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleoredoxin antibody.
❮❯

Immunofluorescent staining of FFPE human Caco-2 cells with Nucleoredoxin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with Nucleoredoxin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal clear cell carcinoma tissue with Nucleoredoxin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) Caco-2, 2) HepG2, 3) HEK293, 4) A549, 5) U-2 OS, 6) A431 and 7) HeLa cell lysate with Nucleoredoxin antibody. Predicted molecular weight ~48 kDa.
Flow cytometry testing of human SiHa cells with Nucleoredoxin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Nucleoredoxin antibody.

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CodeSizePrice
NSJ-RQ7985-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a member of the thioredoxin superfamily, a group of small, multifunctional redox-active proteins. Members of this family are characterized by a conserved active motif called the thioredoxin fold that catalyzes disulfide bond formation and isomerization. The encoded protein acts a redox-dependent regulator of the Wnt signaling pathway and is involved in cell growth and differentiation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E19-I435) was used as the immunogen for the Nucleoredoxin antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q6DKJ4