NSJ Bioreagents

RCOR1 Antibody / REST corepressor 1 / CoREST

Product Code:
 
NSJ-RQ7989
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the CoREST antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human SiHa cells with CoREST antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of human 1) Jurkat, 2) HepG2, 3) HL60 and 4) K562 cell lysate with CoREST antibody. Predicted molecular weight ~53 kDa, commonly observed at 53-66 kDa.
3 / 4
IHC staining of FFPE human esophageal squamous carcinoma tissue with CoREST antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 4
Flow cytometry testing of human K562 cells with CoREST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CoREST antibody.
❮❯

Immunofluorescent staining of FFPE human SiHa cells with CoREST antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Jurkat, 2) HepG2, 3) HL60 and 4) K562 cell lysate with CoREST antibody. Predicted molecular weight ~53 kDa, commonly observed at 53-66 kDa.
IHC staining of FFPE human esophageal squamous carcinoma tissue with CoREST antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human K562 cells with CoREST antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CoREST antibody.

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CodeSizePrice
NSJ-RQ7989-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
REST corepressor 1 also known as CoREST is a protein that in humans is encoded by the RCOR1 gene. This gene encodes a protein that is well-conserved, downregulated at birth, and with a specific role in determining neural cell differentiation. The encoded protein binds to the C-terminal domain of REST (repressor element-1 silencing transcription factor).
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N270-S485) was used as the immunogen for the CoREST antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9UKL0