NSJ Bioreagents

EIF1 Antibody / Eukaryotic translation initiation factor 1

Product Code:
 
NSJ-RQ7993
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Eukaryotic translation initiation factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot testing of 1) human HeLa, 2) human HEK293, 3) human Jurkat, 4) human Raji, 5) human K562, 6) rat pancreas, 7) mouse pancreas and 8) mouse NIH 3T3 cell lysate with Eukaryotic translation initiation factor 1 antibody. Predicted molecular weight ~13 kDa.
3 / 4
Flow cytometry testing of human A431 cells with Eukaryotic translation initiation factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eukaryotic translation initiation factor 1 antibody.
4 / 4
Immunofluorescent staining of FFPE human T-47D cells with Eukaryotic translation initiation factor 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human placental tissue with Eukaryotic translation initiation factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human HEK293, 3) human Jurkat, 4) human Raji, 5) human K562, 6) rat pancreas, 7) mouse pancreas and 8) mouse NIH 3T3 cell lysate with Eukaryotic translation initiation factor 1 antibody. Predicted molecular weight ~13 kDa.
Flow cytometry testing of human A431 cells with Eukaryotic translation initiation factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Eukaryotic translation initiation factor 1 antibody.
Immunofluorescent staining of FFPE human T-47D cells with Eukaryotic translation initiation factor 1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

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NSJ-RQ7993-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Eukaryotic translation initiation factor 1 (eIF1) is a protein that in humans is encoded by the EIF1 gene. It is related to yeast SUI1. In mammalian cells, translation is controlled at the level of polypeptide chain initiation by initiation factors. Eukaryotic translation initiation factor 1 (eIF1) is crucial for the scanning process in vitro. During the scanning process, eIF1 is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of eIF1 in regulating the activity of ribosomal subunits 43S, 48S and 40S. eIF1 enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. It is also a promotor, along with eukar-yotic translation initiation factor 1A (eIF1A), for assembly of 48S ribosomal complexes at the initiation codon of a conventional capped mRNA. In addition, eIF1 and eIF1A, together with eukaryotic translation initiation factor 5 (eIF5), function in the formation of stable 40S ribosomal preinitiation complexes.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids S2-D106) was used as the immunogen for the Eukaryotic translation initiation factor 1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P41567