NSJ Bioreagents

PRPF6 Antibody / Pre-mRNA pr°Cessing factor 6

Product Code:
 
NSJ-RQ7995
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRPF6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with PRPF6 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) rat C6 and 5) mouse NIH 3T3 cell lysate with PRPF6 antibody. Predicted molecular weight ~107 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HeLa cells with PRPF6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF6 antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with PRPF6 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human K562, 3) human 293T, 4) rat C6 and 5) mouse NIH 3T3 cell lysate with PRPF6 antibody. Predicted molecular weight ~107 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with PRPF6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF6 antibody.

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CodeSizePrice
NSJ-RQ7995-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene. The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K61-K585) was used as the immunogen for the PRPF6 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O94906