NSJ Bioreagents

PRPF18 Antibody / Pre-mRNA-splicing factor 18

Product Code:
 
NSJ-RQ8001
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRPF18 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE mouse testis tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE rat testis tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human urothelial carcinoma tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human ovary serous carcinoma tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
Immunofluorescent staining of FFPE human A549 cells with PRPF18 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 8
Western blot testing of 1) human SH-SY5Y, 2) rat brain, 3) rat PC-12 and 4) mouse brain tissue lysate with PRPF18 antibody. Predicted molecular weight ~40 kDa.
7 / 8
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with PRPF18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF18 antibody.
8 / 8
Flow cytometry testing of fixed and permeabilized human JK-1 cells with PRPF18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF18 antibody.
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IHC staining of FFPE mouse testis tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovary serous carcinoma tissue with PRPF18 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A549 cells with PRPF18 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human SH-SY5Y, 2) rat brain, 3) rat PC-12 and 4) mouse brain tissue lysate with PRPF18 antibody. Predicted molecular weight ~40 kDa.
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with PRPF18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF18 antibody.
Flow cytometry testing of fixed and permeabilized human JK-1 cells with PRPF18 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRPF18 antibody.

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CodeSizePrice
NSJ-RQ8001-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E93-L342) was used as the immunogen for the PRPF18 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q99633