NSJ Bioreagents

RIOK2 Antibody / RIO2

Product Code:
 
NSJ-RQ8002
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RIO2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human HL60 cells with RIO2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIO2 antibody.
2 / 2
Western blot testing of human 1) U-251, 2) HeLa and 3) HepG2 cell lysate with RIO2 antibody. Predicted molecular weight ~63 kDa, 55 kDa (two isoforms).

Flow cytometry testing of fixed and permeabilized human HL60 cells with RIO2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RIO2 antibody.
Western blot testing of human 1) U-251, 2) HeLa and 3) HepG2 cell lysate with RIO2 antibody. Predicted molecular weight ~63 kDa, 55 kDa (two isoforms).

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CodeSizePrice
NSJ-RQ8002-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
RIO kinase 2 (RIOK2) is a member of the RIO family of atypical serine protein kinases first characterized in yeast. There are three RIO kinase subfamilies: RIO1, RIO2, and RIO3. RIOK1 and RIOK2 proteins are present in organisms from Archaea to humans. Studies of RIO kinases in yeast indicate that they play a role in ribosome biogenesis. Human RIOK2 has been shown to be a part of a late 40S pre-ribosomal particle and have influence on 40S maturation.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D16-L283) was used as the immunogen for the RIO2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9BVS4