NSJ Bioreagents

Tms1 Antibody / Pycard / mASC

Product Code:
 
NSJ-RQ8006
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Asc antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE mouse RM-1 cells with Asc antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of mouse 1) lung, 2) spleen and 3) thymus tissue lysate with Asc antibody. Predicted molecular weight ~22 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized mouse RAW264.7 cells with Asc antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Asc antibody.
❮❯

Immunofluorescent staining of FFPE mouse RM-1 cells with Asc antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of mouse 1) lung, 2) spleen and 3) thymus tissue lysate with Asc antibody. Predicted molecular weight ~22 kDa.
Flow cytometry testing of fixed and permeabilized mouse RAW264.7 cells with Asc antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Asc antibody.

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CodeSizePrice
NSJ-RQ8006-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
PYCARD, often referred to as ASC (Apoptosis-associated speck-like protein containing a CARD), is a protein that in humans is encoded by the PYCARD gene. This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant mouse protein (amino acids K24-S193) was used as the immunogen for the Asc antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9EPB4