NSJ Bioreagents

PHTF1 Antibody / Putative homeodomain transcription factor 1

Product Code:
 
NSJ-RQ8013
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PHTF1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human U-87 MG cells with PHTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHTF1 antibody.
2 / 2
Western blot testing of human 1) U-87 MG, 2) MCF7, 3) HeLa and 4) SiHa cell lysate with PHTF1 antibody. Predicted molecular weight ~87 kDa but may be observed at higher molecular weights due to glycosylation.
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Flow cytometry testing of fixed and permeabilized human U-87 MG cells with PHTF1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PHTF1 antibody.
Western blot testing of human 1) U-87 MG, 2) MCF7, 3) HeLa and 4) SiHa cell lysate with PHTF1 antibody. Predicted molecular weight ~87 kDa but may be observed at higher molecular weights due to glycosylation.

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NSJ-RQ8013-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
PHTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids I188-Q430) was used as the immunogen for the PHTF1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9UMS5