NSJ Bioreagents

CEA Antibody / Carcinoembryonic Antigen

Product Code:
 
NSJ-RQ8017
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Carcinoembryonic Antigen antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human PC-3 cells with Carcinoembryonic Antigen antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carcinoembryonic Antigen antibody.
2 / 2
Western blot testing of human 1) MCF7 and 2) HaCaT cell lysate with Carcinoembryonic Antigen antibody. Expected molecular weight: 80~200 kDa depending on glycosylation level.
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Flow cytometry testing of fixed and permeabilized human PC-3 cells with Carcinoembryonic Antigen antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Carcinoembryonic Antigen antibody.
Western blot testing of human 1) MCF7 and 2) HaCaT cell lysate with Carcinoembryonic Antigen antibody. Expected molecular weight: 80~200 kDa depending on glycosylation level.

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CodeSizePrice
NSJ-RQ8017-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
CEA (Carcinoembryonic antigen) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. CEA is a glycosyl phosphatidyl inositol(GPI)-cell surface anchored glycoprotein whose specialized sialofucosylated glycoforms serve as functional colon carcinoma L-selectin and E-selectin ligands, which may be critical to the metastatic dissemination of colon carcinoma cells. CEA and related genes make up the CEA family belonging to the immunoglobulin superfamily. In humans, the carcinoembryonic antigen family consists of 29 genes, 18 of which are normally expressed.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids N553-G678) was used as the immunogen for the Carcinoembryonic Antigen antibody.
Purity:
Antigen affinity purified
Uniprot #:
P06731