NSJ Bioreagents

PRKCSH Antibody / Glucosidase 2 subunit beta

Product Code:
 
NSJ-RQ8021
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Glucosidase 2 subunit beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with Glucosidase 2 subunit beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Daudi, 4) human HEL, 5) rat liver, 6) rat L6, 7) mouse liver and 8) mouse C2C12 cell lysate with Glucosidase 2 subunit beta antibody. Predicted molcular weight ~59 kDa but may be observed at higher molecular weights due to glycosylation.
3 / 3
Flow cytometry testing of fixed and permeabilized human SiHa cells with Glucosidase 2 subunit beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glucosidase 2 subunit beta antibody.
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Immunofluorescent staining of FFPE human A549 cells with Glucosidase 2 subunit beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Daudi, 4) human HEL, 5) rat liver, 6) rat L6, 7) mouse liver and 8) mouse C2C12 cell lysate with Glucosidase 2 subunit beta antibody. Predicted molcular weight ~59 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of fixed and permeabilized human SiHa cells with Glucosidase 2 subunit beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glucosidase 2 subunit beta antibody.

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CodeSizePrice
NSJ-RQ8021-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
Glucosidase 2 subunit beta is an enzyme that in humans is encoded by the PRKCSH gene. This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E16-L506) was used as the immunogen for the Glucosidase 2 subunit beta antibody.
Purity:
Antigen affinity purified
Uniprot #:
P14314