NSJ Bioreagents

PLEKHA1 Antibody / TAPP-1

Product Code:
 
NSJ-RQ8032
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TAPP-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
Immunofluorescent staining of FFPE human lung cancer tissue with TAPP-1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 8
IHC staining of FFPE human lung cancer tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human breast cancer tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human placental tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human A431 cells with TAPP-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Western blot testing of human Jurkat cell lysate with TAPP-1 antibody. Predicted molecular weight ~46 kDa.
8 / 8
Flow cytometry testing of fixed and permeabilized human A431 cells with TAPP-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAPP-1 antibody.
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Immunofluorescent staining of FFPE human lung cancer tissue with TAPP-1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE human lung cancer tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation with TAPP-1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human A431 cells with TAPP-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human Jurkat cell lysate with TAPP-1 antibody. Predicted molecular weight ~46 kDa.
Flow cytometry testing of fixed and permeabilized human A431 cells with TAPP-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAPP-1 antibody.

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CodeSizePrice
NSJ-RQ8032-100ug100 ug£535.00
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Pleckstrin homology domain-containing family A member 1, also called Tandem PH domain-containing protein 1 (TAPP-1), is a protein that in humans is encoded by the PLEKHA1 gene. This gene encodes a pleckstrin homology domain-containing adapter protein. The encoded protein is localized to the plasma membrane where it specifically binds phosphatidylinositol 3,4-bisphosphate. This protein may be involved in the formation of signaling complexes in the plasma membrane. Polymorphisms in this gene are associated with age-related macular degeneration. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K76-K387) was used as the immunogen for the TAPP-1 antibody.
Localisation:
Cytoplasmic, nuclear, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
Q9HB21