NSJ Bioreagents

PPM1G Antibody / Protein phosphatase 1G

Product Code:
 
NSJ-RQ8033
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Protein phosphatase 1G antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 11
Immunofluorescent staining of FFPE human lung cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 11
Immunofluorescent staining of FFPE human intestinal cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 11
Immunofluorescent staining of FFPE human esophagus squama cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
4 / 11
Immunofluorescent staining of FFPE human A431 cells with Protein phosphatase 1G antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 11
IHC staining of FFPE human lymphoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human lymphoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human lung cancer tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human breast cancer tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
IHC staining of FFPE human skin squamous carcinoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 11
Western blot testing of human 1) HeLa, 2) 293T, 3) A431 and 4) Jurkat cell lysate with Protein phosphatase 1G antibody. Predicted molecular weight ~59 kDa, commonly observed at 59-75 kDa.
11 / 11
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Protein phosphatase 1G antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protein phosphatase 1G antibody.
❮❯

Immunofluorescent staining of FFPE human lung cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human intestinal cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human esophagus squama cancer tissue with Protein phosphatase 1G antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A431 cells with Protein phosphatase 1G antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human lymphoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin squamous carcinoma tissue with Protein phosphatase 1G antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) 293T, 3) A431 and 4) Jurkat cell lysate with Protein phosphatase 1G antibody. Predicted molecular weight ~59 kDa, commonly observed at 59-75 kDa.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Protein phosphatase 1G antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protein phosphatase 1G antibody.

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CodeSizePrice
NSJ-RQ8033-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Protein phosphatase 1G is an enzyme that in humans is encoded by the PPM1G gene. The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E48-Q457) was used as the immunogen for the Protein phosphatase 1G antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
O15355