NSJ Bioreagents

PGK2 Antibody / Phosphoglycerate kinase 2

Product Code:
 
NSJ-RQ8035
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Phosphoglycerate kinase 2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE mouse RM1 cells with Phosphoglycerate kinase 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
Western blot testing of 1) human HeLa, 2) human HepG2, 3) rat testis, 4) rat brain, 5) rat heart, 6) mouse testis, 7) mouse brain and 8) mouse heart tissue lysate with Phosphoglycerate kinase 2 antibody. Predicted molecular weight ~45 kDa.
3 / 4
Flow cytometry testing of fixed and permeabilized human SiHa cells with Phosphoglycerate kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phosphoglycerate kinase 2 antibody.
4 / 4
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Phosphoglycerate kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phosphoglycerate kinase 2 antibody.
❮❯

Immunofluorescent staining of FFPE mouse RM1 cells with Phosphoglycerate kinase 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) rat testis, 4) rat brain, 5) rat heart, 6) mouse testis, 7) mouse brain and 8) mouse heart tissue lysate with Phosphoglycerate kinase 2 antibody. Predicted molecular weight ~45 kDa.
Flow cytometry testing of fixed and permeabilized human SiHa cells with Phosphoglycerate kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phosphoglycerate kinase 2 antibody.
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with Phosphoglycerate kinase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Phosphoglycerate kinase 2 antibody.

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CodeSizePrice
NSJ-RQ8035-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids K267-K350) was used as the immunogen for the Phosphoglycerate kinase 2 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P07205