NSJ Bioreagents

POLR1B Antibody / DNA-directed RNA polymerase I subunit RPA2

Product Code:
 
NSJ-RQ8040
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the POLR1B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
Immunofluorescent staining of FFPE human PC-3 cells with POLR1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE human breast cancer tissue with POLR1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human lymphoma tissue with POLR1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of human 1) K562, 2) HepG2 and 3) 293T cell lysate with POLR1B antibody. Predicted molecular weight ~128 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human A431 cells with POLR1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= POLR1B antibody.
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Immunofluorescent staining of FFPE human PC-3 cells with POLR1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human breast cancer tissue with POLR1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymphoma tissue with POLR1B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) K562, 2) HepG2 and 3) 293T cell lysate with POLR1B antibody. Predicted molecular weight ~128 kDa.
Flow cytometry testing of fixed and permeabilized human A431 cells with POLR1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= POLR1B antibody.

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CodeSizePrice
NSJ-RQ8040-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
DNA-directed RNA polymerase I subunit RPA2 is an enzyme that in humans is encoded by the POLR1B gene. Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H18-V1135) was used as the immunogen for the POLR1B antibody.
Localisation:
Nuclear, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q9H9Y6