NSJ Bioreagents

BCL11B Antibody / CTIP2

Product Code:
 
NSJ-RQ8042
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the B-cell lymphoma/leukemia 11B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 11
IHC staining of FFPE human chronic tonsillitis tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 11
IHC staining of FFPE human thyroid papillary carcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human placental tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human breast cancer tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human lung adenocarcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human ovary serous carcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human bladder urothelial carcinoma with squamous differentiation tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human lymph node metastasis carcinoma with squamous differentiation tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
Immunofluorescent staining of FFPE human U-2 OS cells with B-cell lymphoma/leukemia 11B antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
Western blot testing of human MOLT4 cell lysate with B-cell lymphoma/leukemia 11B antibody. Predicted molecular weight ~96 kDa but the modified forms of the protein can be observed at ~130 kDa.
11 / 11
Flow cytometry testing of fixed and permeabilized rat C6 cells with B-cell lymphoma/leukemia 11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-cell lymphoma/leukemia 11B antibody.

IHC staining of FFPE human chronic tonsillitis tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid papillary carcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovary serous carcinoma tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human bladder urothelial carcinoma with squamous differentiation tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lymph node metastasis carcinoma with squamous differentiation tissue with B-cell lymphoma/leukemia 11B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with B-cell lymphoma/leukemia 11B antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human MOLT4 cell lysate with B-cell lymphoma/leukemia 11B antibody. Predicted molecular weight ~96 kDa but the modified forms of the protein can be observed at ~130 kDa.
Flow cytometry testing of fixed and permeabilized rat C6 cells with B-cell lymphoma/leukemia 11B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= B-cell lymphoma/leukemia 11B antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ8042-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
B-cell lymphoma/leukemia 11B is a protein that in humans is encoded by the BCL11B gene. This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q10-A628) was used as the immunogen for the B-cell lymphoma/leukemia 11B antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q9C0K0