NSJ Bioreagents

Epcam Antibody

Product Code:
 
NSJ-RQ8046
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Epcam antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE mouse colon tissue with Epcam antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE rat colon tissue with Epcam antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
3 / 6
IHC staining of FFPE mouse colon tissue with Epcam antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat colon tissue with Epcam antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of mouse 1) small intestine and 2) kidney tissue lysate with Epcam antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
6 / 6
Flow cytometry testing of fixed and permeabilized mouse RAW264.7 cells with Epcam antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epcam antibody.
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Immunofluorescent staining of FFPE mouse colon tissue with Epcam antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE rat colon tissue with Epcam antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
IHC staining of FFPE mouse colon tissue with Epcam antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat colon tissue with Epcam antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) small intestine and 2) kidney tissue lysate with Epcam antibody. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Flow cytometry testing of fixed and permeabilized mouse RAW264.7 cells with Epcam antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Epcam antibody.

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CodeSizePrice
NSJ-RQ8046-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant mouse protein (amino acids Q24-A267) was used as the immunogen for the Epcam antibody.
Localisation:
Cell surface, cytoplasmic
Purity:
Antigen affinity purified
Uniprot #:
Q99JW5