NSJ Bioreagents

PRKCA Antibody / PKC alpha

Product Code:
 
NSJ-RQ8049
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRKCA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human SiHa cells with PRKCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCA antibody.
2 / 2
Western blot testing of 1) Jurkat, 2) human 293T, 3) human HeLa, 4) human K562, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human SiHa cells with PRKCA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRKCA antibody.
Western blot testing of 1) Jurkat, 2) human 293T, 3) human HeLa, 4) human K562, 5) rat C6 and 6) mouse NIH 3T3 cell lysate with PRKCA antibody. Predicted molecular weight ~77 kDa.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ8049-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is thought to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PRKCA1 regulates cardiac contractility and propensity toward heart failure.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q142-V672) was used as the immunogen for the PRKCA antibody.
Purity:
Antigen affinity purified
Uniprot #:
P17252