NSJ Bioreagents

HPRT1 Antibody / HGPRTase

Product Code:
 
NSJ-RQ8055
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the HGPRTase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
2 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
3 / 3
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Jurkat, 4) rat brain and 5) mouse brain tissue lysate with HGPRTase antibody. Predicted molecular weight ~24 kDa.
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Flow cytometry testing of fixed and permeabilized human U-2 OS cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
Flow cytometry testing of fixed and permeabilized human 293T cells with HGPRTase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= HGPRTase antibody.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Jurkat, 4) rat brain and 5) mouse brain tissue lysate with HGPRTase antibody. Predicted molecular weight ~24 kDa.

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CodeSizePrice
NSJ-RQ8055-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT, HGPRTase) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Y17-K186) was used as the immunogen for the HGPRTase antibody.
Purity:
Antigen affinity purified
Uniprot #:
P00492