NSJ Bioreagents

PLA2G15 Antibody / LPLA2 / LLPL

Product Code:
 
NSJ-RQ8062
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the LPLA2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human U-2 OS cells with LPLA2 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of human 1) placenta, 2) SiHa and 3) U-251 cell lysate with LPLA2 antibody. Predicted molecular weight ~47 kDa and 36 kDa (two isoforms) but may be observed at higher molecular weights due to glycosylation.
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Immunofluorescent staining of FFPE human U-2 OS cells with LPLA2 antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) placenta, 2) SiHa and 3) U-251 cell lysate with LPLA2 antibody. Predicted molecular weight ~47 kDa and 36 kDa (two isoforms) but may be observed at higher molecular weights due to glycosylation.

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CodeSizePrice
NSJ-RQ8062-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
Lysosomal phospholipase A2, also known as LPLA2, PLA2G15, LLPL and ACS, is an enzyme that in humans is encoded by the PLA2G15 gene. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D92-Q372) was used as the immunogen for the LPLA2 antibody.
Localisation:
Nuclear, cytoplasmic, secreted
Purity:
Antigen affinity purified
Uniprot #:
Q8NCC3