NSJ Bioreagents

PPM1N Antibody / Probable protein phosphatase 1N

Product Code:
 
NSJ-RQ8066
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PPM1N antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with PPM1N antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) RT4, 2) HEL, 3) K562 and 4) A549 cell lysate with PPM1N antibody. Predicted molecular weight ~46 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PPM1N antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPM1N antibody.
❮❯

Immunofluorescent staining of FFPE human A549 cells with PPM1N antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) RT4, 2) HEL, 3) K562 and 4) A549 cell lysate with PPM1N antibody. Predicted molecular weight ~46 kDa.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PPM1N antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPM1N antibody.

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CodeSizePrice
NSJ-RQ8066-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Predicted to enable metal ion binding activity and protein serine/threonine phosphatase activity. Predicted to be involved in negative regulation of I-kappaB kinase/NF-kappaB signaling and positive regulation of canonical Wnt signaling pathway. Predicted to be active in cytosol and nucleus.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R36-A430) was used as the immunogen for the PPM1N antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q8N819