NSJ Bioreagents

NADK2 Antibody / C5orf33

Product Code:
 
NSJ-RQ8067
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NADK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with NADK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HepG2, 2) human HeLa, 3) rat brain, 4) mouse brain and 5) mouse thymus tissue lysate with NADK2 antibody. Predicted molecular weight ~49, 46 and 32 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human HepG2 cells with NADK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NADK2 antibody.
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Immunofluorescent staining of FFPE human A549 cells with NADK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HepG2, 2) human HeLa, 3) rat brain, 4) mouse brain and 5) mouse thymus tissue lysate with NADK2 antibody. Predicted molecular weight ~49, 46 and 32 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human HepG2 cells with NADK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NADK2 antibody.

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CodeSizePrice
NSJ-RQ8067-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes NAD kinase 2, a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D145-Q442) was used as the immunogen for the NADK2 antibody.
Localisation:
Cytoplasmic (mitochondria)
Purity:
Antigen affinity purified
Uniprot #:
Q4G0N4