NSJ Bioreagents

PIGS Antibody / Phosphatidylinositol glycan class S

Product Code:
 
NSJ-RQ8074
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PIGS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human HeLa cells with PIGS antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HCCT, 2) human HCCP, 3) rat liver and 4) rat RH35 cell lysate with PIGS antibody. Predicted molecular weight ~61 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human RT4 cells with PIGS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PIGS antibody.
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Immunofluorescent staining of FFPE human HeLa cells with PIGS antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HCCT, 2) human HCCP, 3) rat liver and 4) rat RH35 cell lysate with PIGS antibody. Predicted molecular weight ~61 kDa.
Flow cytometry testing of fixed and permeabilized human RT4 cells with PIGS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PIGS antibody.

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CodeSizePrice
NSJ-RQ8074-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
GPI transamidase component PIG-S is an enzyme that in humans is encoded by the PIGS gene. This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R184-D504) was used as the immunogen for the PIGS antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
Q96S52