NSJ Bioreagents

Apolipoprotein E Antibody / APOE

Product Code:
 
NSJ-RQ8075
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Apolipoprotein E antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human Caco-2 cell lysate with Apolipoprotein E antibody. Predicted molecular weight: 34-37 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with Apolipoprotein E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Apolipoprotein E antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with Apolipoprotein E antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human Caco-2 cell lysate with Apolipoprotein E antibody. Predicted molecular weight: 34-37 kDa.
Flow cytometry testing of fixed and permeabilized human Caco-2 cells with Apolipoprotein E antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Apolipoprotein E antibody.

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CodeSizePrice
NSJ-RQ8075-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Apolipoprotein E is also known as APOE, AD2 or LPG. The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Alternative splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids E95-Q222) was used as the immunogen for the Apolipoprotein E antibody.
Localisation:
Cytoplasmic, membranous
Purity:
Antigen affinity purified
Uniprot #:
P02649