NSJ Bioreagents

PPP4C Antibody / PP-X

Product Code:
 
NSJ-RQ8106
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PP-X antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 8
IHC staining of FFPE human lung cancer tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 8
IHC staining of FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 8
IHC staining of FFPE human breast cancer with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 8
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 8
IHC staining of FFPE human skin squamous cell carcinoma tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 8
Immunofluorescent staining of FFPE human U-2 OS cells with PP-X antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 8
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) A431 cell lysate with PP-X antibody. Predicted molecular weight ~35 kDa.
8 / 8
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PP-X antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PP-X antibody.
❮❯

IHC staining of FFPE human lung cancer tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human invasive urothelial carcinoma of the bladder with squamous differentiation tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human ovarian serous adenocarcinoma tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human skin squamous cell carcinoma tissue with PP-X antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-2 OS cells with PP-X antibody (red) and Alpha Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) 293T, 3) Jurkat and 4) A431 cell lysate with PP-X antibody. Predicted molecular weight ~35 kDa.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with PP-X antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PP-X antibody.

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CodeSizePrice
NSJ-RQ8106-100ug100 ug£535.00
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This product comes from: United States.
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Serine/threonine-protein phosphatase 4 catalytic subunit, also called Protein phosphatase X (PP-X), is an enzyme that in humans is encoded by the PPP4C gene. Enables protein serine/threonine phosphatase activity. Involved in regulation of double-strand break repair via homologous recombination. Located in cytosol; nucleoplasm; and plasma membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q13-L307) was used as the immunogen for the PP-X antibody.
Localisation:
Cytoplasmic, nuclear
Purity:
Antigen affinity purified
Uniprot #:
P60510