NSJ Bioreagents

SPAG9 Antibody / JIP-4 / JLP

Product Code:
 
NSJ-RQ8107
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the JLP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A431 cells with JLP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human HeLa, 2) human PC-3, 3) rat testis and 4) mouse testis tissue lysate with JLP antibody. Predicted molecular weight ~146 kDa, can be observed at ~190 kDa.
3 / 3
Flow cytometry testing of human HL60 cells with JLP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JLP antibody.
❮❯

Immunofluorescent staining of FFPE human A431 cells with JLP antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human PC-3, 3) rat testis and 4) mouse testis tissue lysate with JLP antibody. Predicted molecular weight ~146 kDa, can be observed at ~190 kDa.
Flow cytometry testing of human HL60 cells with JLP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= JLP antibody.

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CodeSizePrice
NSJ-RQ8107-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
C-jun-amino-terminal kinase-interacting protein 4 (JIP-4), also called JNK-associated leucine-zipper protein (JLP), is a scaffold protein that in humans is encoded by the SPAG9 gene. This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A281-K964) was used as the immunogen for the JLP antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
O60271