NSJ Bioreagents

RAB39A/B Antibody / Ras related protein

Product Code:
 
NSJ-RQ8109
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RAB39A/B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with RAB39A/B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human SH-SY5Y cell lysate with RAB39A/B antibody. Predicted molecular weight ~25 kDa with a possible RAB39A cleavage band also being observed.
3 / 3
Flow cytometry testing of fixed and permeabilized human 293T cells with RAB39A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB39A/B antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with RAB39A/B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human SH-SY5Y cell lysate with RAB39A/B antibody. Predicted molecular weight ~25 kDa with a possible RAB39A cleavage band also being observed.
Flow cytometry testing of fixed and permeabilized human 293T cells with RAB39A/B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RAB39A/B antibody.

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NSJ-RQ8109-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a protein which is a member of the Rab family.The Rab family of proteins is a member of the Ras superfamily of monomeric G proteins. Approximately 70 types of Rabs have now been identified in humans. Rab GTPases regulate many steps of membrane traffic, including vesicle formation, vesicle movement along actin and tubulin networks, and membrane fusion. These processes make up the route through which cell surface proteins are trafficked from the Golgi to the plasma membrane and are recycled. Surface protein recycling returns proteins to the surface whose function involves carrying another protein or substance inside the cell, such as the transferrin receptor, or serves as a means of regulating the number of a certain type of protein molecules on the surface.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-C217) was used as the immunogen for the RAB39A/B antibody.
Localisation:
Cytoplasmic, cell membrane
Purity:
Antigen affinity purified
Uniprot #:
Q14964, Q96DA2