NSJ Bioreagents

PPP2R5D Antibody

Product Code:
 
NSJ-RQ8110
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PPP2R5D antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human SiHa cells with PPP2R5D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R5D antibody.
2 / 2
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human HepG2, 4) rat brain, 5) rat PC-12, 6) mouse brain and 7) mouse Neuro-2a cell lysate with PPP2R5D antibody. Predicted molecular weight ~58 kDa, ~66 kDa and ~70 kDa (multiple isoforms).
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Flow cytometry testing of fixed and permeabilized human SiHa cells with PPP2R5D antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R5D antibody.
Western blot testing of 1) human HeLa, 2) human MCF7, 3) human HepG2, 4) rat brain, 5) rat PC-12, 6) mouse brain and 7) mouse Neuro-2a cell lysate with PPP2R5D antibody. Predicted molecular weight ~58 kDa, ~66 kDa and ~70 kDa (multiple isoforms).

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CodeSizePrice
NSJ-RQ8110-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform is an enzyme that in humans is encoded by the PPP2R5D gene. The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids R91-L602) was used as the immunogen for the PPP2R5D antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q14738