NSJ Bioreagents

E2F1 Antibody

Product Code:
 
NSJ-RQ8118
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the E2F1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of fixed and permeabilized human HeLa cells with E2F1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F1 antibody.
2 / 2
Western blot testing of human 1) Jurkat, 2) HeLa and 3) A431 cell lysate with E2F1 antibody. Expected molecular weight: 48-60 kDa.
❮❯

Flow cytometry testing of fixed and permeabilized human HeLa cells with E2F1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= E2F1 antibody.
Western blot testing of human 1) Jurkat, 2) HeLa and 3) A431 cell lysate with E2F1 antibody. Expected molecular weight: 48-60 kDa.

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CodeSizePrice
NSJ-RQ8118-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Transcription factor E2F1 is a protein that in humans is encoded by the E2F1 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q230-D277) was used as the immunogen for the E2F1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q01094