NSJ Bioreagents

GRP78 Antibody / BiP / HSPA5

Product Code:
 
NSJ-RQ8124
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
After reconstitution, the GRP78 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human HepG2, 4) human T-47D, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with GRP78 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.
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Western blot testing of 1) human placenta, 2) human U-87 MG, 3) human HepG2, 4) human T-47D, 5) rat liver, 6) rat brain, 7) mouse liver and 8) mouse brain tissue lysate with GRP78 antibody. Predicted molecular weight: ~73 kDa, but routinely observed at 70-78 kDa.

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NSJ-RQ8124-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
HSPA5 (heat shock 70kDa protein 5), also known as glucose-regulated protein, 78kD (GRP78), BiP (Binding-immunoglobulin protein), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. GRP78/BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Shen et al. (2002) concluded that GRP78 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of GRP78 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that GRP78 is a key element in sensing the folding capacity within the ER.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D78-S607) was used as the immunogen for the GRP78 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P11021