NSJ Bioreagents

NFRKB Antibody / Nuclear factor related to kappa-B-binding protein

Product Code:
 
NSJ-RQ8132
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the NFRKB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human A549 cells with NFRKB antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) HEL, 2) RT-4, 3) Caco-2 and 4) HaCaT cell lysate with NFRKB antibody. Predicted molecular weight ~139 kDa, commonly observed at ~175 kDa and can be observed at ~220 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HEL cells with NFRKB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NFRKB antibody.
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Immunofluorescent staining of FFPE human A549 cells with NFRKB antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEL, 2) RT-4, 3) Caco-2 and 4) HaCaT cell lysate with NFRKB antibody. Predicted molecular weight ~139 kDa, commonly observed at ~175 kDa and can be observed at ~220 kDa.
Flow cytometry testing of fixed and permeabilized human HEL cells with NFRKB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NFRKB antibody.

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CodeSizePrice
NSJ-RQ8132-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
NFRKB (nuclear factor related to kappa-B-binding protein) was first identified as a factor that could bind a variant NF-kappa B site in the interleukin-2 receptor alpha (IL-2R alpha) gene. NFRKB appears to be a transcription factor central to immune cell development. Evidence of this comes from the identification of NFRKB as an amplified gene in several cases of acute myeloid leukemia. Additionally, in another clinical case, a partial deletion of the chromosomal region that includes NFRKB is shown to be associated with combined cellular immunodeficiency.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H270-L994) was used as the immunogen for the NFRKB antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
Q6P4R8