NSJ Bioreagents

POLR2D Antibody / RNA polymerase II subunit RPB4

Product Code:
 
NSJ-RQ8137
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the POLR2D antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Immunofluorescent staining of FFPE human U-2 OS cells with POLR2D antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 2
Western blot testing of human 1) MCF7 and 2) 293T cell lysate with POLR2D antibody. Predicted molecular weight ~16 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with POLR2D antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF7 and 2) 293T cell lysate with POLR2D antibody. Predicted molecular weight ~16 kDa.

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NSJ-RQ8137-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Direct ELISA: 0.1-0.5ug/ml
Description:
DNA-directed RNA polymerase II subunit RPB4 is an enzyme that in humans is encoded by the POLR2D gene. This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids D7-D124) was used as the immunogen for the POLR2D antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O15514