NSJ Bioreagents

POLR2E Antibody / RPB5

Product Code:
 
NSJ-RQ8138
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the RPB5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with RPB5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human A431, 2) human HeLa, 3) human HepG2, 4) human MCF7 and 5) rat liver tissue lysate with RPB5 antibody. Predicted molecular weight ~24 kDa.
3 / 3
Flow cytometry testing of fixed and permeabilized human HeLa cells with RPB5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPB5 antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with RPB5 antibody (red) and Beta Tubulin mAb (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human A431, 2) human HeLa, 3) human HepG2, 4) human MCF7 and 5) rat liver tissue lysate with RPB5 antibody. Predicted molecular weight ~24 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with RPB5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPB5 antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ8138-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
DNA-directed RNA polymerases I, II, and III subunit RPABC1, also called RPB5 homolog, is a protein that in humans is encoded by the POLR2E gene. This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q95-Q210) was used as the immunogen for the RPB5 antibody.
Localisation:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
P19388