NSJ Bioreagents

PPP2R3A Antibody

Product Code:
 
NSJ-RQ8139
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PPP2R3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human U-2 OS cells with PPP2R3A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human A431 cell lysate with PPP2R3A antibody. Predicted molecular weight ~130 kDa and ~72 kDa as well as possibly ~48 kDa (multiple isoforms).
3 / 3
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with PPP2R3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R3A antibody.
❮❯

Immunofluorescent staining of FFPE human U-2 OS cells with PPP2R3A antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human A431 cell lysate with PPP2R3A antibody. Predicted molecular weight ~130 kDa and ~72 kDa as well as possibly ~48 kDa (multiple isoforms).
Flow cytometry testing of fixed and permeabilized human SH-SY5Y cells with PPP2R3A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP2R3A antibody.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-RQ8139-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Serine/threonine-protein phosphatase 2A regulatory subunit B" subunit alpha is an enzyme that in humans is encoded by the PPP2R3A gene. This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B"/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B" family. The B" family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B". Alternative splicing results in multiple transcript variants encoding different isoforms.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M1-E1150) was used as the immunogen for the PPP2R3A antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q06190