NSJ Bioreagents

PPP3CB Antibody / Calcineurin A beta

Product Code:
 
NSJ-RQ8141
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Monkey
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PPP3CB antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 5
IHC staining of FFPE human rectum adenocarcinoma tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human tonsil tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human urothelial carcinoma tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) human MCF7, 2) monkey COS-7, 3) rat brain, 4) rat heart, 5) rat C6, 6) mouse brain, 7) mouse heart and 8) mouse NIH 3T3 cell lysate with PPP3CB antibody. Predicted molecular weight ~59 kDa.
5 / 5
Flow cytometry testing of fixed and permeabilized human U937 cells with PPP3CB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP3CB antibody.
❮❯

IHC staining of FFPE human rectum adenocarcinoma tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma tissue with PPP3CB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF7, 2) monkey COS-7, 3) rat brain, 4) rat heart, 5) rat C6, 6) mouse brain, 7) mouse heart and 8) mouse NIH 3T3 cell lysate with PPP3CB antibody. Predicted molecular weight ~59 kDa.
Flow cytometry testing of fixed and permeabilized human U937 cells with PPP3CB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PPP3CB antibody.

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CodeSizePrice
NSJ-RQ8141-100ug100 ug£535.00
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  • Further Information
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (PP2BB) is an enzyme that in humans is encoded by the PPP3CB gene. Enables several functions, including calmodulin binding activity; calmodulin-dependent protein phosphatase activity; and protein phosphatase 2B binding activity. Involved in calcineurin-NFAT signaling cascade; positive regulation of transcription by RNA polymerase II; and protein dephosphorylation. Located in cytoplasm. Part of calcineurin complex. Implicated in aortic valve stenosis. Biomarker of focal segmental glomerulosclerosis and schizophrenia.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids H36-Q524) was used as the immunogen for the PPP3CB antibody.
Localisation:
Cytoplasm
Purity:
Antigen affinity purified
Uniprot #:
P16298