NSJ Bioreagents

PRELP Antibody / Prolargin

Product Code:
 
NSJ-RQ8143
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Prolargin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Flow cytometry testing of fixed and permeabilized human RT4 cells with Prolargin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prolargin antibody.
2 / 3
Flow cytometry testing of fixed and permeabilized human HeLa cells with Prolargin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prolargin antibody.
3 / 3
Western blot testing of 1) human HaCaT, 2) human U-251, 3) rat liver and 4) mouse lung tissue lysate with Prolargin antibody. Predicted molecular weight ~44 kDa but may be observed at higher molecular weights due to glycosylation.
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Flow cytometry testing of fixed and permeabilized human RT4 cells with Prolargin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prolargin antibody.
Flow cytometry testing of fixed and permeabilized human HeLa cells with Prolargin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prolargin antibody.
Western blot testing of 1) human HaCaT, 2) human U-251, 3) rat liver and 4) mouse lung tissue lysate with Prolargin antibody. Predicted molecular weight ~44 kDa but may be observed at higher molecular weights due to glycosylation.

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CodeSizePrice
NSJ-RQ8143-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Prolargin is a protein that in humans is encoded by the PRELP gene. The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids I69-R353) was used as the immunogen for the Prolargin antibody.
Purity:
Antigen affinity purified
Uniprot #:
P51888