NSJ Bioreagents

PRTN3 Antibody / Proteinase 3 / Myeloblastin

Product Code:
 
NSJ-RQ8145
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the PRTN3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE human tonsil tissue with PRTN3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human lung adenocarcinoma tissue with PRTN3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human ThP-1 cell lysate with PRTN3 antibody. Predicted molecular weight ~28 kDa but may be observed at higher molecular weights due to glycosylation.
4 / 4
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with PRTN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRTN3 antibody.
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IHC staining of FFPE human tonsil tissue with PRTN3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with PRTN3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human ThP-1 cell lysate with PRTN3 antibody. Predicted molecular weight ~28 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of fixed and permeabilized human ThP-1 cells with PRTN3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRTN3 antibody.

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CodeSizePrice
NSJ-RQ8145-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
Proteinase 3, also known as PRTN3 and Myeloblastin, is an enzyme that in humans is encoded by the PRTN3 gene. Enables enzyme binding activity; serine-type endopeptidase activity; and signaling receptor binding activity. Involved in several processes, including mature conventional dendritic cell differentiation; membrane protein ectodomain proteolysis; and neutrophil extravasation. Located in azurophil granule lumen; cytosol; and plasma membrane raft. Colocalizes with plasma membrane.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids A23-D241) was used as the immunogen for the PRTN3 antibody.
Purity:
Antigen affinity purified
Uniprot #:
P24158