NSJ Bioreagents

Prosaposin Antibody / PSAP

Product Code:
 
NSJ-RQ8146
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Prosaposin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 6
Immunofluorescent staining of FFPE human thyroid cancer with Prosaposin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
2 / 6
Immunofluorescent staining of FFPE human A549 cells with Prosaposin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 6
IHC staining of FFPE human tonsil tissue with Prosaposin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE human thyroid papillary carcinoma tissue with Prosaposin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of human 1) A549, 2) HeLa, 3) HepG2 and 4) MCF7 cell lysate with Prosaposin antibody. Predicted molecular weight ~58 kDa but may be observed at higher molecular weights due to glycosylation. This protein is secreted in its fully glycosylated ~70 kDa form.
6 / 6
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Prosaposin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prosaposin antibody.
❮❯

Immunofluorescent staining of FFPE human thyroid cancer with Prosaposin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
Immunofluorescent staining of FFPE human A549 cells with Prosaposin antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human tonsil tissue with Prosaposin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human thyroid papillary carcinoma tissue with Prosaposin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) HeLa, 3) HepG2 and 4) MCF7 cell lysate with Prosaposin antibody. Predicted molecular weight ~58 kDa but may be observed at higher molecular weights due to glycosylation. This protein is secreted in its fully glycosylated ~70 kDa form.
Flow cytometry testing of fixed and permeabilized human MCF7 cells with Prosaposin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Prosaposin antibody.

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CodeSizePrice
NSJ-RQ8146-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Description:
This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids Q182-I479) was used as the immunogen for the Prosaposin antibody.
Localisation:
Cytoplasmic, secreted
Purity:
Antigen affinity purified
Uniprot #:
P07602